Fig. 8From: A cuproptosis-related gene expression signature predicting clinical prognosis and immune responses in intrahepatic cholangiocarcinoma detected by single-cell RNA sequence analysisVerification of the cuproptosis-related prognosis signature in ICC cells. A mRNA levels of SLC39A4 48 h after transfection. B Proliferation of ICC cells detected by CCK8 assay. C Colony formation assays of ICC cells after siRNA transfection. D Results of migration assays of ICC cells in Transwell plates after siRNA transfection. E Results of ICC cell death after adding TTM. F Protein expression of key genes of cuproptosis after knockdown of SLC39A4 and addition of ESBack to article page