Fig. 5

FLNB contributes to radiation-induced senescence and migration ability of HMVECs. A. qRT-PCR analysis of FLNB expression in HMVECs transiently transfected with si-CTRL or si-FLNB for 24 h, 48 h and 72 h. Samples were run in duplicate. Data were normalized to the GAPDH expression in the corresponding samples. B. WES full-length gel image of FLNB expression in HMVECs transiently transfected with si-CTRL or si-FLNB for 24 h, 48 h and 72 h (left). The relative amount of each immunoreactive band was shown. The protein expression was quantified using AUC (area under the curve) measurements generated using Compass Software (right). Signal intensity was normalized to the vinculin expression in the same sample. C. Measurement of β-gal positive irradiated HMVECs (50 Gy) relative to sham irradiated cells (CTRL, dashed line at value 1). HMVECs transiently transfected with si-CTRL (50 Gy si-CTRL) and HMVECs transiently transfected with si-FLNB (50 Gy si-FLNB) are shown. Results are shown as mean ± SD from two independent experiments. *p < 0.05 vs HMVECs si-CTRL (Student’s t test). D. Scratch-wound assay quantification (left) and images (right) of HMVECs transiently transfected with si-CTRL and HMVECs transiently transfected with si-FLNB at 0 h and 24 h time points. Values are reported as the percentage wound area and shown as mean ± SD from two independent experiments. **p < 0.01 vs HMVECs si-CTRL (Student’s t test) (scale bar: 600 µm; magnification 4X)