Fig. 1

PLK1 directly binds to RhoGDI1 in vitro and in vivo. a 293 T cells were co-transfected with HA-tagged PLK1 and Flag-tagged RhoGDI1. Cell lysates were immunoprecipitated with HA (left) or Flag antibody (right). Immunoprecipitates and total lysates were immunoblotted with HA and Flag antibodies. b Cell lysates from HeLa were immunoprecipitated with IgG (as control) or PLK1 antibodies, and immunoblotted with PLK1 or RhoGDI1 antibodies. c Proximity ligation assay (PLA) was performed with PLK1 and RhoGDI1 antibodies in HeLa cells. Representative fluorescence images show blue (DAPI) staining nuclei and red dots (PLA signals). Scale bars: 50 μm. d His-tagged PLK1 and GST-tagged RhoGDI1 were expressed in E.coli. GST pull-down assay was performed with His-PLK1 expression lysates and purified GST-RhoGDI1. The GST pull-down products were immunoblotted with PLK1 and GST antibodies