Fig. 5

Interaction between PLK1 and RhoGDI1 is required for the migration and invasion of cancer cells. a Western blot analysis of PLK1 and RhoGDI1 expression in human cancer cell lines. b Indicated cells were transfected with GFP or GFP-RhoGDI1 aa 90–111. Cell proliferation was assessed by counting the viable cells after trypan blue staining at each day point. c, d Indicated cells were transfected with GFP or GFP-RhoGDI1 aa 90–111. 24 h after transfection, cells were serum-starved overnight and then subjected to migration c and invasion d assay as described in Materials and Methods. Representative images of migrating or invading cells stained with crystal violet were displayed (left). The relative percentages of migrating or invading cells were quantified as described in Materials and Methods (right). Quantitative data display the mean ± SD (n = 3). e 2 × 10.⁶ HeLa cells that overexpress GFP (control) or GFP-RhoGDI1 aa 90-111 were injected via the tail vein of female BALB/c nude mice (n = 6 per group). Representative image of H&E stained lung tissue from HeLa-GFP (control) or HeLa-GFP-RhoGDI1 aa 90-111 (left). The number of metastatic nodules was counted per lung section and displayed in the scatter plots (right). Quantitative data display the mean ± SD (n = 6). Scale bar = 500 μm. **P < 0.01