Fig. 6

The synthetic peptide containing RhoGDI1 aa 90-111 inhibits cancer cell migration and invasion. a FITC-labeled synthetic peptide sequence. b Cellular uptake of FITC-labeled peptides by HeLa cells. Scale bar = 50 μm. Data are representative of three independent experiments. c HeLa cells were transfected with Flag-RhoGDI1 and treated with 20 μM peptides for 12 h. The lysates were immunoprecipitated with Flag antibody. Immunoprecipitates and total lysates were immunoblotted with the indicated antibodies. d, e Indicated cells were treated with 20 μM peptides and subjected to migration (d) and Invasion (e) assay. Representative images of migrating or invading cells (left). The relative percentages of migrating or invading cells (right). Quantitative data display the mean ± S.D. (n = 3). *P < 0.05; **P < 0.01