Fig. 1

Downregulation of hsa-circ-0013561 inhibits HNSCC cell proliferation and tumor growth. A, B FISH detection showing the expression and subcellular distribution of hsa-circ-0013561. C qRT-PCR detection showing the expression of hsa-circ-0013561 in Hep-2 cells after transduction with NC or sh-circ0013561. ^***p < 0.001 vs. si-NC. D CCK-8 detection showing Hep-2 cell proliferation. ^***p < 0.001 vs. NC. E, F EdU assay showing Hep-2 proliferation after transduction with NC or sh-circ0013561. ^**p < 0.01 vs. NC. G, H Colony formation assay showing Hep-2 proliferation after transduction with NC or sh-circ0013561. ^**p < 0.01 vs. NC. All data are expressed as the mean ± SD. I Representative images of HNSCC tumor formation in nude mouse xenograft models. J Summary of mouse tumor volumes measured every three days. ***p < 0.001 vs. sh-NC. K, L Tumor volumes and weights measured 25 days post-injection. **p < 0.01, ***p < 0.001 vs. NC. M, N Immunohistochemistry showing the percentage of Ki67-positive cells. ***p < 0.001 vs. NC. All data are presented as the mean ± SD. All experiments were repeated three times