Fig. 3

HNSCC cell migration and invasion are inhibited with hsa-circ-0013561 downregulation. A, B Transwell assay showing the migration of Hep-2 cells after silencing hsa-circ-0013561. ^**p < 0.01 vs NC. C, D Wound healing assay showing the invasion of Hep-2 cells after silencing hsa-circ-0013561. ^****p < 0.0001 vs NC. E, F Western blot for epithelial-to-mesenchymal transition proteins E-cadherin and N-cadherin. **p < 0.01, *p < 0.05 vs. NC. All data are presented as the mean ± SD. G, H Live imaging showing HNSCC cell pulmonary metastases in mice. The data are expressed as the mean ± SD. ^*p < 0.05 vs NC. I, J The number of metastatic foci in lung tissues were determined using hematoxylin and eosin staining. K, L Live imaging showing HNSCC cells lymphatic metastases. The data are expressed as the mean ± SD. ***p < 0.001 vs NC. M Immunohistochemistry for CD31 staining in lymphatic tissue from mouse models. All experiments were repeated three times