Fig. 5

miR-7-5p and PDK3 are downstream targets of hsa-circ-0013561. A Prediction of binding sites of miR-7-5p to hsa-circ-0013561. The mutant (MUT) version of hsa-circ-0013561 is presented. B Relative luciferase activity 48 h after transduction of Hep-2 cells with miR-7-5p mimic/NC or wild-type (WT)/Mut hsa-circ-0013561-overexpressing lentivirus. **p < 0.01. C Prediction of binding sites of miR-7-5p to the 3′-UTR of PDK3. The MUT version of 3′-UTR-PDK3 is shown. D Relative luciferase activity determined 48 h after transduction of Hep-2 cells with miR-7-5p mimic/NC or 3′-UTR-PDK3 WT/Mut-overexpressing lentivirus. **p < 0.01. All data are presented as mean ± SD. E, F qRT-PCR data showing hsa-circ-0013561 and miR-7-5p expression of Hep-2 cells. ^*p < 0.05, ^***p < 0.001 vs. NC. G qRT-PCR and western blot showing the expression of PDK3. ^****p < 0.0001 vs. NC. All data are presented as mean ± SD