Fig. 2

DEGs between patients with high and low YY1 expression. (A) YY1 expression in tumor and adjacent gastric tissues (***p < 0.001, Paired and Non-paired t test). (B) qRT-PCR analysis of YY1 mRNA expression and Western blot of YY1 expression (C) 150-paired gastric cancer and adjacent normal gastric tissues identified YY1 mRNA (**p < 0.01, Paired t test) and 50-paired tissue protein expression(*p < 0.001, Non-paired t test) showed YY1 is significantly elevated in gastric tumor tissue. (D) A total of 4386 significant differentially expressed genes (DEGs) were detected between GC patients with high and low YY1 expressions. (E) A total of 4741 YY1 co-expressed genes were downloaded from the cbioportal. (F) A total of 105 transcriptional targets and 11 transcription factors related to YY1 were predicted by the TRRUST v2 database. (G) 30 genes were identified on the Venn diagram of the intersection between DEGs, co-expressed genes, regulators, and targets of YY1. (H) KEGG enrichment analysis on the 30 hub genes. (I) GSEA combining all DEGs, co-expressed genes, regulators, and targets of YY1. TP53_ACTIVITY_THROUGH_PHOSPHORYLATION, REACTOME_SIGNALING_BY_TGFB_FAMILY_MEMBERS, and REACTOME_GLYCEROPHOSPHOLIPID_BIOSYNTHESIS were the significantly enriched pathways regulated by YY1