Fig. 5

Effects of YY1 overexpression and knockdown on cell proliferation and by CCK-8 in HGC-27 and MFC cells. (A-B) After YY1 overexpression, the cell lines tumors presented a significant elevation on cell proliferation and drug resistance for Apatinib. The overexpression of YY1 led enhanced the invasion ability (**p < 0.001, non-paired t test) capacities by transwell assays in HGC-27 (C) and MFC (D) cells. (E) Effects of YY1 overexpression on tumor growth curve for subcutaneous tumors derived from cells infected with the lentivirus encoding YY1. After YY1overexpression, the subcutaneous tumors presented a significant drug resistance for both Apatinib and the mouse PD-1 antibody. The treatment with mouse IFN-α significantly reversed both Apatinib and mPD-1 antibody resistance of YY1 overexpressed subcutaneous tumors (**p < 0.001, Non-paired t test, Scale bar: 5 mm). (F) After treatment with mouse IFN-α, both CD8 and CD27 were significantly upregulated among GC tissues, indicating an improvement of the immune microenvironment of subcutaneous GC tissues (View: 200X and 400X)