Fig. 4

Analysis of cell cycle arrest and cellular senescence. A–B Flow cytometry was used to analyze the effect of PD2 on the cell cycle of HCC cells; C–D Quantitative PCR was used to analyze the effect of PD2 on the SASP of HCC cells. E Western blotting of cell cycle- and senescence-related proteins; (F–H) The changes in cell senescence induced by PD2 were analyzed by β-galactosidase staining and immunofluorescence detection of Lamin B1 and γ-H2A.X. The scale bar equals 50 F–G and 10 H µm. Compared with the control group, * * * P < 0.001, **P < 0.01 and *P < 0.05