Fig. 5

Analysis of key genes causing cell senescence. A The inhibitory effect of PD2 on HCCLM3 cells was analyzed after silencing P21 or overexpressing Cyclin A2; B After silencing P21 and overexpressing Cyclin A2, the expressions of cell proliferation-related and cell senescence-related proteins were measured; C–D The changes in cell cycle were measured after P21 silencing and Cyclin A2 overexpression. E Cell senescence was measured by β-galactosidase staining after silencing P21 or overexpressing Cyclin A2; F The expression of P21 and Cyclin A2 after NIX/BNIP3L silencing; G Cell cycle changes were observed after NIX/BNIP3L silencing. The scale bar equals 50 µm. Compared with the control group, * * * P < 0.001, * * P < 0.01 and *P < 0.05