Fig. 3

Phf6 deletion suppresses MLL::AF9-induced AML progression. (A) Experimental scheme of establishing Phf6 KO AML model; (B) Phf6 deletion was confirmed by molecular gel electrophoresis of DNA. n = 3; (C-D) Survival analysis of mice transplanted with 1 × 10⁵ (C) and 2 × 10⁵ GFP⁺ cells (D). n = 6, significance differences were calculated with the log-rank test; (E) The frequency of GFP⁺ cells in PB was examined at different times post pIpC. The first arrow represents when the PB GFP⁺ cells reached 3.5% and pIpC injection. The second arrow represents when the PB GFP⁺ cells reached 10% and pIpC injection. n = 3, Student’s t-test; (F) Wright–Giemsa staining of PB cells; (G) The expression level of Mac-1⁺ Gr-1⁺ in GFP⁺ cells. n = 3, Student’s t-test; (H) Hematoxylin-eosin (HE) staining of BM, spleen, and liver. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001