Fig. 6

On TNBC cells, 3-MA reversed the effects of ICA. A, B MDA-MB-468 and 4T1 cells were treated with ICA (25 µM) with or without 3-MA (5 mM). Apoptosis was analyzed using flow cytometry. C, D Western blotting showing the expression of P62, BECN1, and LC3B proteins in MDA-MB-468 and 4T1 after ICA with or without 3-MA exposure, with β-actin as an internal control. Bars represent the means ± SD of at least three independent experiments; *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the control group (0 µM). TNBC triple-negative breast cancer, 3-MA 3-methyladenine, ICA icariin, P62 SQSTM1, BECN1 Beclin1, LC3B MAP1LC3B