Fig. 7

ICA inhibited tumor growth and promoted autophagy in vivo. A Image of 4T1 tumors from different groups. B Average tumor weight of each group. Bars represent the means ± SD of six mice. C The growth of 4T1 xenograft tumors during treatment with control (vehicle), ICA (20 mg/kg), and ICA (40 mg/kg). Bars represent the means ± SD of six mice. D Changes in body weight of mice in different groups during treatment. Bars represent the means ± SD of six mice. E H&E staining of tumor, liver, kidney, and spleen tissues in each group. F, G Ki-67, P62 and LC3B immunohistochemical results of tumors from each group. Bars represent the means ± SD of three independent experiments. H, I The protein expression of p-mTOR, mTOR, p-ULK1, ULK1, p-AMPK, and AMPK in xenograft tumor. Bars represent the means ± SD of three mice tumor samples. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the control group (0 µM). ICA icariin, TNBC triple-negative breast cancer, H&E hematoxylin and eosin