Fig. 4

SLC25A17 knockdown induces autophagy in TNBC cells. A Expression levels of P62, Beclin-1 and LC3-I, LC3-II in SLC25A17 knockdown or overexpression cells were detected by western blotting. B Representative LC3 immunofluorescence staining using an anti-LC3 antibody in SLC25A17 knockdown cells. (scale bar: 100 μm). C Representative images of MDC staining for autophagosomes in SLC25A17 knockdown cells. (scale bar: 100 μm). D Detection of autophagic flux by the mRFP-GFP-LC3 lentivirus in SLC25A17 knockdown MDA-MB-231 cells with confocal microscopy. (scale bar: 10 μm). E The number of autophagosomes was detected by electron microscopy in SLC25A17 knockdown MDA-MB-231 cells, and the autophagosomes were indicated by red arrows. (scale bar: 2 μm). F Western blot analysis of LC3-I to LC3-II conversion in SLC25A17 knockdown cells with or without chloroquine (CQ, 20 µM)