Fig. 6

ANP32B effects MYC expression in AML cell lines. A Volcano plot analysis revealed differentially expressed genes obtained from RNA-seq data between the ANP32B knockdown and the control groups. B After ANP32B knockdown, MV4-11 and Kasumi-1 cells exhibited enrichment of down-regulated genes, resulting in 1473 common genes being identified, including MYC. C GSEA plots demonstrated gene enrichment in HALLMARK_MYC signaling pathways in AML cells treated with ANP32B knockdown. D KEGG pathway enrichment analysis. E Heatmap view displayed the top downregulated genes in MV4-11 and Kasumi-1 cells treated with ANP32B knockdown. F Western blotting analysis showed that the C-MYC, ANP32B, CCNB1, CDK2, BCL-2 and PLK1 were downregulated in MV4-11 and Kasumi-1 cells after ANP32B knockdown. G ANP32B knockdown led to downregulate of MYC, CCNB1, CDK2, BCL-2 and PLK1 in MV4-11 and Kasumi-1 cells, determined using qRT-PCR