Fig. 6

The oncogenic role of KCNE4 in CAFs. (a) Spearman’s correlation analysis was utilized to determine the correlation between the expression levels of FAP, VIM, FSP1, ACTA2, and KCNE4, with the data from the GEPIA database. (b) Immunoblot Analysis of CAFs Markers in NAFs with Induced KCNE4 overexpression. (c) The effect of KCNE4 on NAFs proliferation was determined by EdU incorporation assay. (d) Impact of KCNE4 on NAFs migration revealed via transwell Migration. (e) The protein levels of CAFs markers in CAFs transfected with KCNE4 siRNAs. (f) EdU assay for detecting proliferation CAFs Proliferation following KCNE4 siRNA transfection. (g) Migration of CAFs transfected with KCNE4 siRNAs. (h) Cell adhesion assay of LoVo- and HCT116-GFP cells on CAFs transfected with KCNE4 siRNA-NC or siRNA-KCNE4. (i) Migration of LoVo and HCT116 cells incubated with CM derived from CAFs transfected with KCNE4 siRNA-NC or siRNA-KCNE4. (j) The mRNA expression levels of the target genes of multiple chemokine and cytokine. (k) Analysis of the correlation between KCNE4 and IGF1 from the TCGA database. Data in bar graphs indicate mean ± SEM. **P < 0.01, ***P < 0.001, ****P < 0.0001. Student’s t test (c, d), multi-group analysis of variance (f, g, h, i, j)