Fig. 3

Effects of LASS2 overexpression on ferroptosis in different tumours. A Venn diagram of 183 overlapping signalling pathways enriched in LASS2-overexpressing cells according to LC–MS analysis. B Important signalling pathways based on Venn diagrams. C The ferroptosis signalling pathway was enriched based on Gene set enrichment analysis (GSEA) from LASS2 overexpression (FDR q = 0.019, NES = -1.543, P = 0.019). D–G Transcriptomics (D) and proteomics (F) analyses of the enriched KEGG pathways. GSEA in (E) and (G) reveals enriched pathways for differentially expressed genes. H–J Morphological changes in mitochondria in three tumour cell lines were observed via transmission electron microscopy. The typical features are indicated by the yellow arrows. K MDA content was measured by a colorimetric assay after LASS2 overexpression in the BCPAP, MDA-MB-231, and Hepa1-6 cell lines. L, M Mitochondrial ROS levels measured by flow cytometry. **P < 0.01, ***P < 0.001