Fig. 4

The interaction between LASS2 and TFRC. A Network interaction maps constructed using STRING software (https://cn.string-db.org/) for enriched ferroptosis-related proteins in the tumour cell lines revealed by LC–MS. (Only some of the proteins are presented.) B The Venn diagram with TFRC in the intersection of the three tumours. C Prediction of LASS-TFRC protein binding by ZDOCK 3.0.2. D The binding mode of the LASS2-TFRC complex was predicted via ZDOCK 3.0.2, and its structural domains were identified via UniProt (https://www.uniprot.org/). E Detection of the colocalization of endogenous LASS2 with TFRC by immunofluorescence in the BCPAP, MDA-MB-231, and Hepa1-6 cell lines (scale bar: 100/50 μm). F–H Interaction of LASS2 with TFRC, revealed by co-IP and western blotting in three cell lines. I–K The direct interaction between LASS2 and TFRC was confirmed by PLA