Fig. 1
From: Micropeptides: potential treatment strategies for cancer
Several techniques for the identification of micropeptides. (A) Ribosome sequencing: identifying sORFs with coding potential. (B) Liquid chromatography-tandem mass spectrometry (LC-MS/MS) provides reliable evidence for the presence of sORF-encoded peptides (SEPs). (C) CRISPR-Cas9: Determining the function of micropeptides by knocking down the sORF of non-canonical peptides. (D) In vitro translation: assessing the encoding capacity of Sorf. (E) Bioinformatics analysis: algorithmic analysis and database search to obtain potential micropeptides. (F) Western Blot:Perform micropeptide coding and functional validation