From: Micropeptides: potential treatment strategies for cancer
Techniques | Advantages | Disadvantages | Reference |
---|---|---|---|
Ribosome sequencing | Accurate identification of translation start sites | False positives can be produced due to nonspecific binding | Â |
Enables protein synthesis processes monitoring in vivo | rRNA and tRNA produce Ribo-Seq noise | 39,40 | |
Useful to investigate extension speed, cotranslation processing, etc | Since RNase digests rRNA, it can damage ribosome integrity and cause experimental bias | Â | |
Mass spectrometry | Direct verification of the protein-coding potential of transcripts | Small protein can be lost during sample preparation and purification | Â |
 | Depends on data from protein databases | 45,46 | |
Bioinformatics | A wide range of emerging tools enables, choices to best meet the need | Micropeptides are easy to miss due to their small size | Â |
 | Experiments are needed to confirm the translatability of the identified sORFs | 51,52 | |
Other experimental verification | Direct verification of micropeptides | The experimental process is tedious | Â |
Determination of the subcellular location of micropeptides and micropeptide interactions with other macromolecules | Â | 6,53,57 |