Fig. 2

RGS20 promotes NSCLC cell proliferation and activates autophagy in vitro A Cell proliferation of three different groups (CTRL, RGS20-KD, and RGS20-OE) in H1299 and Anip973 cells was detected by the Cell Counting Kit-8 (CCK-8) assay. B Colony formation assays show the colony-forming ability of H1299 and Anip973 cells in three groups (CTRL, RGS20-KD, and RGS20-OE). The number of colonies was counted and is displayed on the right side. C Representative western blot results show the protein levels of autophagy-related proteins and the corresponding quantification analysis. D Representative western blot results show the protein levels of autophagy related proteins after blockage of autophagy flux by Baf-A1 (50 nM, 2 h) in three groups (CTRL, RGS20-KD, and RGS20-OE) of H1299 and Anip973 cells. The corresponding quantification analysis results were listed on the right. E CCK-8 assay was performed to assess the proliferation of H1299 and Anip973 cells in the RGS20-OE group treated with Baf-A1 (50 nM, 2 h). F Colony formation assays were used to analyze the colony-forming ability of H1299 and Anip973 cells in the RGS20-OE group treated with Baf-A1 (50 nM, 2 h). The number of colonies was counted and is displayed on the right side. * p < 0.05, **p < 0.01, ***p < 0.001