Fig. 3

RGS20 inhibits the Hippo signaling pathway in NSCLC A KEGG pathway analysis was performed by transcriptome sequencing to identify the signaling pathway in which differentially expressed genes were enriched. B The representative Western blot result shows the protein levels of p-YAP and YAP in the control, RGS20-KD, and RGS20-OE groups of H1299 and Anip973 cells. The corresponding quantification analysis results are shown on the right. C Representative immunofluorescence images show the nuclear translocation of YAP in three different groups of H1299 and Anip973 cells. D The representative Western blot result shows the p-YAP and YAP protein levels after the blockage of the Hippo signaling pathway with GA-017 (10 μM, 24 h) in the RGS20-KD and control groups. The corresponding quantification analysis results are shown on the right. E Representative immunofluorescence images of YAP in the RGS20-KD group after treatment with GA-017. F CCK-8 assay to detect the proliferation of H1299 and Anip973 cells after treatment with GA-017 (10 μM, 72 h) in the control and RGS20-KD groups. G Colony formation assays were used to analyze the colony-forming ability of H1299 and Anip973 cells treated with GA-017 (10 μM, 72 h). H Representative Western blot result shows autophagy-related protein levels after the blockage of the Hippo signaling pathway with GA-017 (10 μM, 24 h) in the RGS20-KD and control groups. * p < 0.05, **p < 0.01, ***p < 0.001