Fig. 4

RGS20 promotes cell proliferation and enhances autophagy by inhibiting the PKA-Hippo signaling pathway in vitro A Representative western blot result shows the protein level of p-PKA and PKA in RGS20-OE cells after treatment with forskolin (10 μM, 72 h). The corresponding results of the quantification analysis are displayed on the right. B Representative western blot result shows the protein level of p-YAP and YAP after the activation of the PKA signaling pathway with forskolin (10 μM, 72 h) in RGS20-OE and control cells. C Representative immunofluorescence images of YAP in the RGS20-OE group after treatment with forskolin. D The CCK-8 assay detects the proliferation of H1299 and Anip973 cells after treatment with forskolin (10 μM, 72 h) in the RGS20-OE and control groups. E Colony formation assays were used to analyze the colony-forming ability of H1299 and Anip973 cells after treatment with forskolin (10 μM, 72 h). F Representative western blot result shows the protein levels of autophagy-related proteins after the activation of the PKA signaling pathway with forskolin (10 μM, 72 h) in the RGS20-OE and control groups. * p < 0.05, **p < 0.01, ***p < 0.001