Fig. 5

RGS20 enhances the tumorigenesis of NSCLC cells in vivo A Tumor-promoting effects of RGS20 in a xenograft mouse model. The representative images of tumor-bearing mice that were photographed at the end of the third week of experimentation. B Representative images of the dissected tumors. A ruler was used to indicate the size of the tumors. C The tumor growth curve was plotted using xenograft tumor volume data. D Tumor weight was measured after tumor excision. E Representative images of HE staining and IHC staining of Ki-67 and RGS20. Representative western blot results show the protein level of the PKA-Hippo (F, G) signaling pathway and autophagy-related proteins (H). The corresponding quantification analysis results are listed below. Protein levels were normalized to GAPDH. Results are presented as the mean ± SD (n = 3). * p < 0.05, **p < 0.01, ***p < 0.001