Fig. 2
SorafenibR and parental Hep3b/Huh7 cells show different DNA methylation potential. a and b, qPCR (a) or Western blotting (b) measuring expression levels of DNMT1, DNMT3a, DNMT3b, HDAC2 and HDAC3. Data are shown as mean values ± SD. c and d, Dotblotting measuring changes in DNA methylation using 5mC antibody (c). The graphs (d) show the quantification of dot intensity as mean values ± S.D. from three independent experiments. e and f, qPCR (e) or Western blotting (f) measuring expression levels of TET1, TET2, and TET3. Data are shown as mean values ± SD. g and h, Dotblotting measuring changes in DNA hydroxymethylcytosine using 5hmC antibody (g). The graphs (h) show the quantification of dot intensity as mean values ± S.D. from three independent experiments. *P < 0.05; ns, not significant; LC, loading control