Fig. 3
Role of DNMT3a and TET2 in resistant cell growth. SorafenibR Hep3b and Huh7 cells were infected with DNMT3a or TET2 shRNA or control viruses and selected by 2 µg/ml puromycin for 5 days. a and b, qPCR (a) and Western blotting (b) assessing efficacy of DNMT3a knockdown. Data represents three independent experiments, and the graphs are shown as mean values ± S.D. c and d, Dotblotting measuring changes of DNA methylation or hydroxymethylcytosine using 5mC and 5hmC antibodies. The graphs (d) are the quantification of dot intensity shown as mean values ± S.D. from three independent experiments. e and f, Proliferation (e) and migration (f) assays in resistant cells 5 days after virus infection in drug-free medium. Data represents two independent experiments with 12 repeats in total. g and h, qPCR (g) and Western blotting (h) showing TET2 knockdown. Data represents three independent experiments. i and j, Dotblotting measuring changes in DNA methylation or hydroxymethylcytosine using 5mC and 5hmC antibodies (i). The graphs (j) are the quantification of dot intensity as mean values ± S.D. from three independent experiments. k and l, Proliferation (k) and migration (l) assays in resistant cells 5 days after virus infection in drug-free medium. Data represents two independent experiments with 12 repeats in total. *P < 0.05; ns, not significant