Fig. 1

Domperidone effectively inhibits proliferation of ESCC cells

(A) Domperidone was identified through soft agar assay from the clinical drug library. (B) The chemical structure of Domperidone is depicted. (C) KYSE150, KYSE450 and SHEE cells were treated with different doses of domperidone (0, 5, 10, 25, 50 100 and 200 µM) for 48 h, and IC 50 calculated by GraphPad Prism 8. (D) The human ESCC cell lines, KYSE150 (left panel) and KYSE450 (right panel), were exposed to various concentrations of domperidone or DMSO as control group for 24 h, 48 h, 72 and 96 h. The cell numbers were calculated and analyzed by IN Cell Analyzer 6000. (E) Anchorage-independent cell growth was assessed using a soft agar assay with different concentrations of domperidone (0, 10, 20, 40 and 60 µM). After incubation for 10 days, colonies were captured and analyzed using In Cell Analyze 6000. Results from three independent experiments were presented by comparing domperidone treatment group with control group. (F) Representative images obtained from the clone formation assay are shown. (G) KYSE150 (Left) and KYSE450 (right) were treated with 40 µM domperidone for 24 h, and then cell proliferation was detected by EdU staining. Data were showed as mean values ± SD. *P < 0.05, **P < 0.01, ***P < 0.001