Fig. 2

Phosphoproteomics analysis of domperidone inducing molecular changes. (A) Schematic representation of phosphorylated proteomics analysis in KYSE150 cells treated with 40 µM domperidone after 24 h. (B) Quantitative proteins were presented as a volcano plot, where down-regulated proteins are represented by green plots and up-regulated proteins by red plots. (C) KEGG pathway enrichment analysis was performed to identify down-regulated proteins in domperidone treatment group compared to control group. (D) A network illustrating major was presented, with circles representing down-regulated proteins after domperidone treatment. (E) Western blot results confirmed down regulation of phosphorylation sites, including p-SMAD3^T8, p-ERK2^T185/Y187 and p-JUND^S100