Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: Domperidone inhibits cell proliferation via targeting MEK and CDK4 in esophageal squamous cell carcinoma

Fig. 3

Domperidone directly binds to MEK1/2 and CDK4

(A) Docking model analysis illustrated the interaction between domperidone and MEK1/2 or CDK4. (B) KYSE150 or (C) KYSE450 cell lysate was incubated with domperidone-conjugated Sepharose 4B beads, MEK1/2 and CDK4 were pulled down through visualization using Western blot. Immunoblot analysis of pronase-digested KYSE150 (D) and KYSE450 (E) cell lysate showed the presence of MEK1/2 and CDK4. (F) Pull down assay was used to evaluate the binding affinity between domperidone and mutant MEK1. (G) Pull down assay was used to evaluate the binding affinity between domperidone and mutant MEK2. (H) Pull down assay was used to evaluate the binding affinity between domperidone and mutation CDK4.

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us