Fig. 4

Number of macrophages per single colorectal cancer cell at depth 4 and depth 5. A The representative CD68-immunohistochemistry (IHC) in colorectal cancer (CRC) at depth 4; Eight CD68-positive (CD68⁺) macrophages (Mφs) infiltrated to depth 4 in this case. Bar, 50 μm. B The representative CD68-immunohistochemistry (IHC) in colorectal cancer (CRC) at depth 5; Fifty CD68⁺ Mφs infiltrated to a depth of 5 in this case. Bar, 50 μm. C Histological spatial analysis identified CRC cells as a red color at depth 4; One hundred twenty-five CRC cells were observed at depth 4 in this case. Bar, 50 μm. D Histological spatial analysis identified CRC cells as a red color at depth 5; Forty CRC cells were observed at depth 5 in this case. Bar, 50 μm. E Comparison between the number of CD68⁺Mφs per single CRC cell at depth 4 and that at depth 5; based on the representative figure A and C, the number of CD68⁺ Mφs per single CRC cell at depth 4 was calculated to be 0.064 (8/125) in this case., and the mean ± SD in 20 cases was 0.69 ± 0.45. Based on the representative figure B and D, the number of CD68⁺ Mφs per single CRC cell at depth 5 was calculated to be 1.25 (50/40) in this case, and the mean ± SD in 20 cases was 2.56 ± 2.19. the Boxplot showed that the number of CD68⁺Mφs per single CRC cell at depth 5 was statistically higher than that at depth 4 (P < 0.01). F Comparison between the number of CD163⁺Mφs per single CRC cell at depth 4 and that at depth 5; the Boxplot showed that the number of CD163⁺Mφs per single CRC cell at depth 5 was statistically higher than that at depth 4 (n = 20; depth 4, 1.78 ± 1.57; depth 5, 5.31 ± 4.30; P < 0.01). G Comparison between the number of CD204⁺Mφs per single CRC cell at depth 4 and that at depth 5; the Boxplot showed that the number of CD204⁺Mφs per single CRC cell at depth 5 was statistically higher than that at depth 4 (n = 20; depth 4, 0.22 ± 0.29; depth 5, 6.70 ± 4.48; P < 0.01). H Comparison between the number of CD206⁺Mφs per single CRC cell at depth 4 and that at depth 5; the Boxplot showed that the number of CD206⁺Mφs per single CRC cell at depth 5 was statistically higher than that at depth 4 (n = 20; depth 4, 0.10 ± 0.24; depth 5, 1.34 ± 3.74; P < 0.01)