Fig. 3

WFDC21P interacted with SEC63 to regulate calcium homeostasis signaling pathway. (A) Top five proteins that interacted with WFDC21P were predicted by catRAPID database. (B) RIP assays using anti-SEC63 antibody were performed to identify the interaction between WFDC21P and SEC63 protein. (C) Biotin labed WFDC21P sequence was used to performed RNA pull down. Western blotting was used to verify the enrichment of SEC63. (D) WFDC21P expression after inhibiting SEC63. (E) The mRNA level of SEC63 after inhibiting WFDC21P expression. (F) Western blotting assay was used to measure the SEC63 expression after WFDC21P downregulation. (G) KEGG chart showed the calcium homeostasis signaling pathway regulated by SEC63. (H) The key protein levels in calcium homeostasis signaling pathway were detected by western blotting after WFDC21P downregulation and SEC63 overexpression. The data are presented as the mean ± SD of at least three independent experiments. ns, not significant; **, p < 0.01