Fig. 6
From: The upregulation of VGF enhances the progression of oral squamous carcinoma
Clinical and functional implications of IL23R expression. (A) IL23R transcripts as related to clinicopathological parameters in our OSCC tumors. X-axis clinicopathological states. Y-axis, TPM of IL23R. (B – E) Exogenous IL23R expression rescues VGF-associated oncogenicity in OSCC cells. (B) qPCR analysis. Duplicate analysis, statistical analysis not performed. (C - E) Phenotypes. (C) Growth. A two-way ANOVA test is performed to evaluate the difference in growth in the exponential growth phase between day 2 and day 4 in SAS cells and between day 3 and day 5 in FaDu cells. The original growth curves are shown in Fig. S6. (D) Migration, (E) Invasion. Lt, SAS cell. Rt, FaDu cell. VGF, VGF expression cell subclones established by the transfection of pBabe-puroVGF plasmid and selection with puromycin. Con, Control cell subclones. IL23R, Transfection of pcDNA3.1(-)IL23R plasmid. VA, vector alone transfection