Fig. 9

Multiple validation that ACACA promotes tumor progression in vivo and in vitro. A, B Expression of ACACA in HCC patient samples and SD rat orthotopic liver cancer model. Bar, 100 µm. Zoom bar, 50 µm. C, D Expression of ACACA in HCC cell lines. Bar, 25 µm. E Expression of ACACA in HCC stem cell and their parental cell. F CCK8 detection: the effect of ACACA knockout on the sensitivity of sorafenib. G, H Edu and colony forming assay: the effect of ACACA knockdown on the proliferation of HCC cell lines. Bar, 50 µm. I Sphere enrichment assay: the effect of ACACA knockdown on the stemness traits of HCC cell lines. Bar, 50 µm. J Wound healing assay: the effect o of ACACA knockdown on the migration capability of HCC cell lines. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns no statistical significance. n = 3 per group