Fig. 3

Pathway enrichment of DEGs and identification of m6A-gene-clusters. (A) Circo-plot of KEGG and GO analysis of 147 DEGs. Left: genes; Right: pathways. Different colors represent different pathways. (B) 3 significant clusters in protein-protein interaction network of 147 DEGs. Rectangles represent protein. Edges represent protein-protein associations. (C) Consensus clustering analysis identification of two clusters (samples, n = 669). The white (consensus value = 0, samples never clustered together) and blue (consensus value = 1, samples always clustered together) heatmap display sample consensus. (D) heatmap of unsupervised clustering of 147 DEGs. Yellow represented high expression of regulators and blue represented low expression. (E) The differential expression of 23 m6A regulators between m6A-gene-clusters. m6A-gene-clusterA, Grey; m6A-gene-clusterB, Red. The upper and lower ends of the boxes represented interquartile range of values. The lines in the boxes represented median value, and black dots showed outliers. (F) Survival plot of m6A-gene-clusters (m6A-gene-clusterA:337 HCCs; m6A-gene-clusterB: 332 HCCs). The m6A-gene-clusterB showed better overall survival than m6A-gene-clusterA (P = 0.007; HR = 1.49). (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001)