Fig. 6

Characteristics of m6A score in prognosis, immune microenvironment and metabolism. (A) Survival plot of m6A score (High-m6A score group:335 HCCs; Low-m6A score group: 334 HCCs). The high-m6A score group showed better overall survival than the low- m6A score group (P = 0.0003; HR = 1.69). (B) Differences in m6Ascore between m6A-gene-clusters. The Mann-Whitney U test was used to compare the statistical difference between m6A-gene-clusters. High-m6A score group, Grey; Low-m6A score group, Red. The upper and lower ends of the boxes represented interquartile range of values. The lines in the boxes represented median value, and black dots showed outliers. The asterisks represented the statistical p value. (C) Differences in m6Ascore between m6A modification patterns (Mann-Whitney U test). (D) Differences in m6Ascore among different tumor stages (Mann-Whitney U test). (E) Differences in m6Ascore between TP53-mutation and TP53-wide type, vascular invasion and non-vascular invasion (Mann-Whitney U test). (F) Validation: Survival plot of m6A score in GSE14520 cohort. The high-m6A score group showed better recurrence-free survival than the low- m6A score group (P = 0.011; HR = 1.62). (G) Survival plot of m6A score in GSE14520 cohort. The high-m6A score group showed better overall survival than the low- m6A score group (P = 0.035; HR = 1.61). (H) Relationship between m6A score and TNM stage, BCLC stage, CLIP stage, tumor number, tumor size in validation cohort (GSE14520). (I) The differential immune cell infiltration between high-m6A score group and low-m6A score group. (J, K) Differences in expression of TGFβ and CYT (cytolytic activity) between high-m6A score group and low-m6A score group. (L) Spearman correlation between m6A score and expression of CTLA4. P < 0.0001. (M) Comparison of glucose metabolism related genes between high-m6A score group and low-m6A score group. (Heatmap; Yellow represented high expression of regulators and blue represented low expression). (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001)