Fig. 5

C6orf15 can affect long-chain fatty acid metabolism in CRC through WNT/β-catenin signalling. a The GSEA-Hallmark database indicates a strong correlation between C6orf15 and fatty acid oxidation. b Control and C6orf15-OE RKO cells were cultured in substrate-limited medium, followed by FAO testing using the Seahorse XF96 Cell Output Analyser as described in the Materials and Methods section. Etomoxir, oligo, FCCP, and antimycotic A (anti-A) were added sequentially as indicated by the dotted line. OCR measurements were normalized to the total number of cells. c Fatty acid-driven mitochondrial respiration was calculated from OCR measurements and used to reflect basal and maximal levels of fatty acid oxidation. The results are shown as the mean ± SD (*p < 0.05). d Western blots show the protein expression of CPT1A in C6orf15-overexpressing CRC and C6orf15-silenced CRC. For all statistical results, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and ns (no statistical significance)