Fig. 4

Utilizing single-cell RNA sequencing analysis for the identification of immune cell populations. A–B Illustrate the relative proportions of each cell type in the public dataset and the integrated immune cell proportions in the EMTAB8107 dataset. C–D Employ the unified flow approximation and projection (UMAP) technique to visually represent BRCA cells, color-coded based on main cell types and malignancy. E Visualizes the expression clusters of MCU through UMAP plots, subsequently subjected to gene set enrichment analysis (GSEA) for F TGFβ, G interferon γ, and H PI3K/AKT/mTOR signaling. I The heatmap depicts the expression of the MCU gene and cell type biomarkers in different single-cell type clusters of tissues. J Highlights CD8Tex cells as key regulators of transcription factors in this cell cluster