Fig. 7
Gene expression in BRCA defined by spatial transcriptomics. A Utilizing spatial transcriptomics, tissue sections were analyzed to identify clusters, accurately aligning them with morphological features observed in hematoxylin and eosin staining and cluster mapping. Malignant areas are outlined by yellow dotted lines, and magnified images of gene variants in boxes i and ii are presented. B–C Spatial distribution and genetic changes involving MCU, AHR, FOXP3, ID2, IL10, IL21, IRF4, TGFb1, and STAT4 in different tissue sections were visualized using 10 × Visium Spatial Gene Expression. Bars indicate tumor versus non-tumor transcript levels. D Dot plots illustrate gene expression levels across various clusters. *P < 0.05