Fig. 2

Knockdown of CDCA5 inhibits the proliferation and migration ability of ccRCC cells in vitro. A, B RT-qPCR (A) and WB (B) analyses of CDCA5 protein expression when knocking down of CDCA5 through lentivirus (CDCA5 shRNA). NC, negative control; KD, CDCA5 knockdown. C Cell survival assay with a fluorescence microscope in 786-O or ACHN cells with or without CDCA5 KD at indicated time points. D, E MTT analysis of cell proliferation in 786-O (D) or ACHN (E) cells with or without CDCA5 KD. F Annexin V and propidium iodide staining was performed to evaluated cell apoptosis of 786-O cells, 786-O cells with CDCA5 KD, as well as ACHN cells, ACHN cells with CDCA5 KD. G–J Wound healing (G, H) and cell migration (I, J) experiments to evaluate the effect of CDCA5 on 786-O and ACHN cell metastasis in vitro. **P < 0.01 and ***P < 0.001. NC, negative control; KD knockdown