Fig. 5

PI3K/AKT/mTOR signaling is essential for CDCA5 mediated ccRCC cell proliferation. A, B WB analysis of p-AKT, P-S6, P-4EBP1, CDCA5 and β-actin levels in ACHN or 786-O with or without CDCA5 knockdown (KD) or CDCA5 overexpression (OE). The expression intensity was compared using arbitrary ratios normalized against β-actin. C MTT analysis of the cell proliferation of ACHN NC, and ACHN OE cells with or without Torin1 (100 nM) treatment for 72 h. Data show mean ± SD from three biological replicates