Fig. 7

EEF1A1 mediates the impact of CDCA5 on ccRCC cell proliferation and migration via mTOR pathway. A EEF1A1 were overexpressed in ACHN cells with CDCA5 knockdown. WB analysis of CDCA5, EEF1A1, P-S6, P-4EBP1 and β-actin levels in ACHN cells, and CDCA5 KD ACHN cells with or without EEF1A1 overexpression (EEF1A1 OE). B, C Cell proliferation was evaluated by MTT assay in ACHN cells, CDCA5 KD ACHN cells with or without EEF1A1 OE (B), as well as 786-O cells, CDCA5 KD 786-O cells with or without EEF1A1 OE (C). D Cell migration ability was also determined via transwell assay in ACHN cells, CDCA5 KD ACHN cells with or without EEF1A1 OE. E Quantification of cell migration in Fig. 7D. ***P < 0.001