Fig. 2

Treatment with T. gondii reduces the proliferation in breast cancer cells. A The cell morphology of MCF-7cells after 48 h of infection with T. gondii at 400 magnification. ME49-MCF7: MCF-7 cell infected with T. gondii ME49 strain (MOI = 16); RH-MCF7: MCF-7 cell infected with T. gondii RH strain (MOI = 4). Arrows indicate extracellular tachyzoites. B The cell morphology of MDA-MB-231 cells after 48 h of infection with T. gondii at 400 magnification. ME49-MDA: MDA-MB-231 cell infected with T. gondii ME49 strain (MOI = 16); RH-MDA: MDA-MB-231 cell infected with T. gondii RH strain (MOI = 4). Arrows indicate extracellular tachyzoites. C Bar graphs showing the decreased mRNA levels of Ki67 in MCF-7 cells treated with T. gondii compared to controls, as determined by qRT-PCR. D Bar graphs showing the decreased mRNA levels of Ki67 in MDA-MB-231 cells treated with T. gondii compared to controls, as determined by qRT-PCR. Data are presented as mean ± SD. ns: non-significant; *: P < 0.05; **: P < 0.01; ***: P < 0.001