Fig. 3
Treatment with T. gondii reduces the migration in breast cancer cells. A Cell wound healing assay showed that migration ability of MCF-7 cells was suppressed by T. gondii. MCF-7 cells (2 × 106 cells/well) were infected with T. gondii ME49 strain (MOI = 16) and RH strain (MOI = 4), and the scratch changes were observed at 0, 6, 12 and 24 h. B Quantification of cellular migration via measurement of wound healing rate in MCF-7 cells infected with T. gondii as represented in A. C Cell wound healing assay showed that migration ability of MDA-MB-231 cells was suppressed by T. gondii. MDA-MB-231 cells (2 × 106 cells/well) were infected with T. gondii ME49 strain (MOI = 16) and RH strain (MOI = 4), and the scratch changes were observed at 0, 6, 12 and 24 h. D Quantification of cellular migration via measurement of wound healing rate in MDA-MB-231 cells infected with T. gondii as represented in C. Images obtained at indicated times were representatives of three independent experiments. E Bar graphs showing the decreased mRNA levels of E-cadherin in MCF-7 cells treated with T. gondii compared to controls, as determined by qRT-PCR. F Bar graphs showing the decreased mRNA levels of E-cadherin in MDA-MB-231 cells treated with T. gondii compared to controls, as determined by qRT-PCR. Bars represent mean ± SD (n = 3). ns: non-significant; *: P < 0.05; **: P < 0.01; ***: P < 0.001