Fig.4

Dual RNA-seq on the T. gondii and breast cancer cell after their interaction. A Schematic of the experiment representing the main step of the infection with T. gondii and the time points when RNA was extracted. Libraries were created and processed through high-throughout sequencing. Reads were assigned to either the Homo sapines or T. gondii transcriptome, and DEGs were detected by DESeq2. B PCA of the breast cancer cells triplicate results for each group. Each replicate is represented by a circle. Each group was assigned a colour: orange (RH-MDA), light blue (RH-MCF7), yellow (ME49-MDA), dark green (ME49-MCF7), red (MDA-control), purple (MCF7-control). Note: two of the replicates of the ME49-MCF7 group were overlapped. C PCA of the T. gondii RH strain triplicate results for each group. Each replicate is represented by a circle. Each group was assigned a colour: orange (RH-MDA), light green (T. gondii RH). D PCA of the T. gondii ME49 strain triplicate results for each group. Each replicate is represented by a circle. Each group was assigned a colour: yellow (ME49-MDA), dark blue (T. gondii ME49)