Table 3 The hub genes regulated by T. gondii in MCF-7 cells were validated by qRT-PCR
Genes | Hazard ratio (95% CI)a | qRT-PCR (relative expression) | RNA-seq (FPKM) | ||||
|---|---|---|---|---|---|---|---|
Controls | ME49-infected | RH-infected | Controls | ME49-infected | RH-infected | ||
Genes that are up-regulated by T. gondii | |||||||
 MYC | 0.59 (0.47, 0.74) | 1.01 | 3.59 | 3.24 | 37.14 | 119.43 | 85.44 |
 RPS5 | 0.69 (0.55, 0.87) | 1.00 | 1.18 | 1.34 | 656.63 | 1171.19 | 990.45 |
 ICAM1 | 0.70 (0.55, 0.89) | 1.03 | 63.84 | 5.45 | 1.21 | 56.06 | 4.08 |
 EGR1 | 0.59 (0.47, 0.74) | 1.01 | 7.07 | 8.08 | 9.66 | 33.78 | 39.39 |
 SOD2 | 0.77 (0.60, 0.98) | 1.02 | 10.03 | 2.25 | 28.55 | 163.03 | 48.52 |
 BNIP3 | 0.70 (0.56, 0.89) | 1.02 | 3.23 | 5.20 | 24.26 | 76.55 | 101.14 |
 RPS12 | 0.72 (0.57, 0.90) | 1.01 | 1.67 | 1.73 | 424.46 | 842.89 | 649.95 |
 AHCY | 0.54 (0.43, 0.67) | 1.01 | 1.48 | 1.86 | 116.95 | 219.62 | 212.79 |
 JUNB | 0.73 (0.58, 0.91) | 1.02 | 2.27 | 3.02 | 74.25 | 173.24 | 133.80 |
 NME2 | 0.53 (0.42, 0.66) | 1.02 | 1.45 | 1.70 | 1093.00 | 2030.18 | 1671.80 |
Genes that are down-regulated by T. gondii | |||||||
 KI67 | 1.49 (1.19, 1.87) | 1.00 | 0.83 | 0.81 | 31.75 | 11.23 | 15.02 |
 RACGAP1 | 1.60 (1.26, 2.02) | 1.04 | 0.49 | 0.50 | 92.84 | 35.89 | 44.23 |
 PRC1 | 1.71 (1.37, 2.14) | 1.01 | 0.64 | 0.70 | 108.63 | 46.79 | 55.05 |
 RAD21 | 1.35 (1.07, 1.70) | 1.01 | 0.90 | 0.99 | 134.41 | 50.67 | 73.46 |
 DHX15 | 1.58 (1.26, 2.00) | 1.03 | 1.02 | 0.98 | 63.73 | 33.28 | 41.67 |
 RPS6KB1 | 1.55 (1.24, 1.94) | 1.04 | 0.81 | 1.04 | 201.17 | 69.72 | 122.90 |