Toxoplasma gondii suppresses proliferation and migration of breast cancer cells by regulating their transcriptome

Table 4 The hub genes regulated by T. gondii in MDA-MB-231 cells were validated by qRT-PCR

Genes	Hazard ratio (95% CI)^a	qRT-PCR (relative expression)			RNA-seq (FPKM)
Genes	Hazard ratio (95% CI)^a	Controls	ME49-infected	RH-infected	Controls	ME49-infected	RH-infected
Genes that are up regulated by T. gondii
IL6	0.79 (0.63, 0.99)	1.01	18.88	5.58	90.33	138.15	217.73
EGR1	0.59 (0.47, 0.74)	1.05	162.30	66.92	1.64	10.19	40.00
CDKN1A	0.58 (0.46, 0.73)	1.02	7.26	3.17	97.48	229.97	211.58
JUNB	0.73 (0.58, 0.91)	1.13	13.63	9.39	24.83	47.98	58.85
CCL3L1	0.72 (0.56, 0.93)	1.06	123.60	114.80	29.99	73.42	140.15
ID3	0.71 (0.56, 0.90)	1.03	0.99	2.13	10.73	34.02	24.27
G enes that are down regulated by T. gondii
PRC1	1.71 (1.37, 2.14)	1.02	0.24	0.84	83.91	48.207	54.31

^a These results were from the KM plotter database. The low expression group was used as the reference group

ISSN: 1475-2867