Fig. 3
Effect of P2X7R SNPs on calcium transients in PANC-1, PSCs and HEK293. a Representative recordings of Ca2+ signals (Fura-2 ratio) in P2X7R + GFP PANC-1 and PSCs (top and bottom panels) transfected with WT (blue), Gly150Arg (orange) and Arg276His (magenta) receptor. The graphs show the response after stimulation with BzATP 100 µM (grey arrow) and the positive control ionomycin 1 µM (green arrow). b Area under the curve (AUC) calculated for 600 s (PANC-1), 400 s (PSCs) and 160 s (HEK293) after the response starts. c Change in Fura-2 (ΔFura-2) between the basal and the maximum peak response to the agonist. Each point represents the change of Fura-2 in a single cell. Comparison between two variants have been evaluated with the non-parametric Mann–Whitney test and the p values are reported in the graphs. Each graph in (b, c) shows responses of cells in 3–4 independent experiments and medians and the interquartile range