Fig. 4

Reduced ATP-induced dye uptake in PANC-1, PSCs and HEK293 cells expressing Gly150Arg and Arg276His. a Original single-cell traces of the ATP-induced YO-PRO-1 uptake in P2X7R + GFP PANC-1 (top) and PSCs (bottom) expressing WT (blue), Gly150Arg (orange) and Arg276His (magenta) receptors. YO-PRO-1 was added after 25 min (beige arrow) prior to stimulation with 5 mM ATP (green arrow). b Comparison of AUC of P2X7R + GFP expressing the different P2X7R variants. Statistical significance was evaluated with the non-parametric Mann–Whitney test and the p values are reported in the graphs. Graphs in (b) and (c) include results from single cells obtained in 3–4 independent experiments